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Alterations in integrin expression modulates invasion of pancreatic cancer cells

Walsh, Naomi and Clynes, Martin and Crown, John and O'Donovan, Norma (2009) Alterations in integrin expression modulates invasion of pancreatic cancer cells. Journal of Experimental & Clinical Cancer Research, 28 (140). ISSN 1756-9966

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Abstract

Background Factors mediating the invasion of pancreatic cancer cells through the extracellular matrix (ECM) are not fully understood. Methods In this study, sub-populations of the human pancreatic cancer cell line, MiaPaCa-2 were established which displayed differences in invasion, adhesion, anoikis, anchorage-independent growth and integrin expression. Results Clone #3 displayed higher invasion with less adhesion, while Clone #8 was less invasive with increased adhesion to ECM proteins compared to MiaPaCa-2. Clone #8 was more sensitive to anoikis than Clone #3 and MiaPaCa-2, and displayed low colony-forming efficiency in an anchorage-independent growth assay. Integrins beta 1, alpha 5 and alpha 6 were over-expressed in Clone #8. Using small interfering RNA (siRNA), integrin β1 knockdown in Clone #8 cells increased invasion through matrigel and fibronectin, increased motility, decreased adhesion and anoikis. Integrin alpha 5 and alpha 6 knockdown also resulted in increased motility, invasion through matrigel and decreased adhesion. Conclusion Our results suggest that altered expression of integrins interacting with different extracellular matrixes may play a significant role in suppressing the aggressive invasive phenotype. Analysis of these clonal populations of MiaPaCa-2 provides a model for investigations into the invasive properties of pancreatic carcinoma.

Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:integrins; pancreatic cancer; invasion; siRNA;
Subjects:Biological Sciences > Cell biology
Medical Sciences > Cancer
DCU Faculties and Centres:Research Initiatives and Centres > National Institute for Cellular Biotechnology (NICB)
Publisher:BioMed Central
Official URL:http://www.jeccr.com/content/28/1/140
Copyright Information:© 2009 Walsh et al.
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
Funders:PRTL1 Cycle 3 and 4 programme of the Higher Education Authority.
ID Code:14942
Deposited On:13 Oct 2009 14:13 by Naomi Walsh. Last Modified 13 Oct 2009 14:13

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