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Nickel(II)-catalysed oxidative guanine and DNA damage beyond 8-oxoguanine

Kelly, Michele C. and Whitaker, Gillian and White, Blánaid and Smyth, Malcolm R. (2007) Nickel(II)-catalysed oxidative guanine and DNA damage beyond 8-oxoguanine. Free Radical Biology and Medicine, 42 (11). pp. 1680-1689. ISSN 0891-5849

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Abstract

Oxidative DNA damage is one of the most important and most studied mechanisms of disease. It has been associated with a range of terminal diseases such as cancer, heart disease, hepatitis, and HIV, as well as with a variety of everyday ailments. There are various mechanisms by which this type of DNA damage can be initiated, through radiation and chemical oxidation, among others; however, these mechanisms have yet to be fully elucidated. A HPLC-UV-EC study of the oxidation of DNA mediated by nickel(II) obtained results that show an erratic, almost oscillatory formation of 8-oxoguanine (8-oxoG) from free guanine and from guanine in DNA. Sporadic 8-oxoG concentrations were also observed when 8-oxoG alone was subjected to these conditions. A HPLC-MS/MS study showed the formation of oxidised-guanidinohydantoin (oxGH) from free guanine at pH 11, and the formation of guanidinohydantoin (GH) from DNA at pH 5.5.

Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:Nickel; HPLC-UV-EC; HPLC-MS/MS; Oxidative DNA damage; Guanine; 8-Oxo-7,8-dihydroguanine;
Subjects:Medical Sciences > Diseases
Physical Sciences > Chemistry
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Chemical Sciences
Research Initiatives and Centres > National Centre for Sensor Research (NCSR)
Publisher:Elsevier
Official URL:http://dx.doi.org/10.1016/j.freeradbiomed.2007.02.025
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
ID Code:150
Deposited On:21 Jan 2008 by DORAS Administrator. Last Modified 02 Feb 2009 11:32

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