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Monolithic centrifugal microfluidic platform for bacteria capture and concentration, lysis, nucleic-acid amplification, and real-time detection

Garcia-Cordero, Jose L. and Dimov, Ivan K. and O'Grady, J. and Ducrée, Jens and Barry, T. and Ricco, Antonio J. (2009) Monolithic centrifugal microfluidic platform for bacteria capture and concentration, lysis, nucleic-acid amplification, and real-time detection. In: MEMS 2009 - 22nd IEEE International Conference on Micro Electro Mechanical Systems, 25-29 January 2009 , Sorrento, Italy. ISBN 978-1-4244-2978-3

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Abstract

We report the design, fabrication, and characterization of a polymer centrifugal microfluidic system for the specific detection of bacterial pathogens. This single-cartridge platform integrates bacteria capture and concentration, supernatant solution removal, lysis, and nucleic-acid sequence-based amplification (NASBA) in a single unit. The unit is fabricated using multilayer lamination and consists of five different polymer layers. Bacteria capture and concentration are accomplished by sedimentation in five minutes. Centrifugation forces also drive the subsequent steps. A wax valve is integrated in the cartridge to enable high-speed centrifugation. Oil is used to prevent evaporation during reactions requiring thermal cycling. Device functionality was demonstrated by real-time detection of E. coli from a 200-muL sample.

Item Type:Conference or Workshop Item (Paper)
Event Type:Conference
Refereed:Yes
Uncontrolled Keywords:bioMEMS; biochemistry; biomedical equipment; macromolecules; microchannel flow; microfabrication; microorganisms; molecular biophysics; multilayers; polymer films; sedimentation;
Subjects:Biological Sciences > Microfluidics
DCU Faculties and Centres:Research Initiatives and Centres > Biomedical Diagnostics Institute (BDI)
Published in:Proceedings of the 22nd IEEE International Conference on Micro Electro Mechanical Systems. . Institute of Electrical and Electronics Engineers. ISBN 978-1-4244-2978-3
Publisher:Institute of Electrical and Electronics Engineers
Official URL:http://dx.doi.org/10.1109/MEMSYS.2009.4805392
Copyright Information:©2009 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE.
Funders:Science Foundation Ireland
ID Code:15460
Deposited On:14 Jul 2010 14:46 by DORAS Administrator. Last Modified 14 Jul 2010 14:46

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