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Design and fabrication of a centrifugally driven microfluidic disk for fully integrated metabolic assays on whole blood

Steigert, J. and Brenner, T. and Grumann, M. and Riegger, L. and Zengerle, R. and Ducrée, Jens (2006) Design and fabrication of a centrifugally driven microfluidic disk for fully integrated metabolic assays on whole blood. In: MEMS 2006 - 19th IEEE International Conference on Micro Electro Mechanical Systems, 22-26 January 2006 , Istanbul, Turkey. ISBN 0-7803-9475-5

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Abstract

For the first time, we present a novel and fully integrated centrifugal microfluidic “ lab-on-a-disk” for rapid metabolic assays in human whole blood. All essential steps comprising blood sampling, metering, plasma extraction and the final optical detection are conducted within t = 150 s in passive structures integrated on one disposable disk. Our technology features a novel plasma extraction structure (V = 500 nL, CV < 5%) without using any hydrophobic microfluidics where the purified plasma (cRBC< 0.11%) is centrifugally separated and subsequently extracted through a capillarily primed extraction channel into the detection chamber. While this capillary extraction requires precisely defined, narrow micro-structures, the reactive mixing and detection is most efficient within larger cavities. The corresponding manufacturing technique of these macro- and micro structures in the range of 30 µ m to 1000 µ m is also presented for the first time: A novel, cost-efficient hybrid prototyping technique of a multiscale epoxy master for subsequent hot embossing of polymer disks.

Item Type:Conference or Workshop Item (Paper)
Event Type:Conference
Refereed:Yes
Subjects:Biological Sciences > Microfluidics
DCU Faculties and Centres:Research Initiatives and Centres > Biomedical Diagnostics Institute (BDI)
Published in:Proceedings of the 19th IEEE International Conference on Micro Electro Mechanical Systems. . Institute of Electrical and Electronics Engineers. ISBN 0-7803-9475-5
Publisher:Institute of Electrical and Electronics Engineers
Official URL:http://dx.doi.org/10.1109/MEMSYS.2006.1627825
Copyright Information:©2006 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE.
ID Code:15470
Deposited On:14 Jul 2010 14:28 by DORAS Administrator. Last Modified 14 Jul 2010 14:28

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