Design and fabrication of a centrifugally driven microfluidic disk for fully integrated metabolic assays on whole blood
Steigert, J., Brenner, T., Grumann, M., Riegger, L., Zengerle, Roland and Ducrée, JensORCID: 0000-0002-0366-1897
(2006)
Design and fabrication of a centrifugally driven microfluidic disk for fully integrated metabolic assays on whole blood.
In: MEMS 2006 - 19th IEEE International Conference on Micro Electro Mechanical Systems, 22-26 January 2006, Istanbul, Turkey.
ISBN 0-7803-9475-5
For the first time, we present a novel and fully integrated centrifugal microfluidic “ lab-on-a-disk” for rapid metabolic assays in human whole blood. All essential steps comprising blood sampling, metering, plasma extraction and the final optical detection are conducted within t = 150 s in passive structures integrated on one disposable disk. Our technology features a novel plasma extraction structure (V = 500 nL, CV < 5%) without using any hydrophobic microfluidics where the purified plasma (cRBC< 0.11%) is centrifugally separated and subsequently extracted through a capillarily primed extraction channel into the detection chamber. While this capillary extraction requires precisely defined, narrow micro-structures, the reactive mixing and detection is most efficient within larger cavities. The corresponding manufacturing technique of these macro- and micro structures in the range of 30 µ m to 1000 µ m is also presented for the first time: A novel, cost-efficient hybrid prototyping technique of a multiscale epoxy master for subsequent hot embossing of polymer disks.
Proceedings of the 19th IEEE International Conference on Micro Electro Mechanical Systems.
.
Institute of Electrical and Electronics Engineers. ISBN 0-7803-9475-5