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Parallelization of chip-based fluorescence immuno-assays with quantum-dot labelled beads

Grumann, M. and Riegger, L. and Nann, T. and Riegler, J. and Ehlert, O. and Mittenbuhler, K. and Urban, G. and Pastewka, L. and Brenner, T. and Zengerle, R. and Ducrée, Jens (2005) Parallelization of chip-based fluorescence immuno-assays with quantum-dot labelled beads. In: TRANSDUCERS 2005 - 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 5-9 June 2005, Seoul, Korea. ISBN 0-7803-8994-8

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Abstract

This paper presents an optical concept for the read-out of a parallel, bead-based fluorescence immunoassay conducted on a lab-on-a-disk platform. The reusable part of the modular setup comprises a detection unit featuring a single LED as light source, two emission-filters, and a color CCD-camera as standard components together with a spinning drive as actuation unit. The miniaturized lab-on-a-disk is devised as a disposable. In the read-out process of the parallel assay, beads are first identified by the color of incorporated quantum dots (QDs). Next, the reaction-specific fluorescence signal is quantified with FluoSpheres-labeled detection anti-bodies. To enable a fast and automated read-out, suitable algorithms have been implemented in this work. Based on this concept, we successfully demonstrated a Hepatitis-A assay on our disk-based lab-on-a-chip.

Item Type:Conference or Workshop Item (Paper)
Event Type:Conference
Refereed:Yes
Uncontrolled Keywords:biomedical optical imaging; fluorescence; readout electronics; semiconductor quantum dots;
Subjects:Biological Sciences > Microfluidics
DCU Faculties and Centres:Research Initiatives and Centres > Biomedical Diagnostics Institute (BDI)
Published in:TRANSDUCERS 2005 - 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. . Institute of Electrical and Electronics Engineers. ISBN 0-7803-8994-8
Publisher:Institute of Electrical and Electronics Engineers
Official URL:http://dx.doi.org/10.1109/SENSOR.2005.1497271
Copyright Information:©2005 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE.
ID Code:15476
Deposited On:14 Jul 2010 14:21 by DORAS Administrator. Last Modified 14 Jul 2010 14:21

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