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Cellular tracking in time-lapse phase contrast images

Thirusittampalam, K. and Hossain, Mohammad J. and Ghita, Ovidiu and Whelan, Paul F. (2009) Cellular tracking in time-lapse phase contrast images. In: IMVIP 2009 - 13th International Machine Vision and Image Processing Conference, 2-4 September 2009 , Dublin, Ireland. ISBN 978-1-4244-4875-3

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Abstract

The quantitative analysis of live cells is a key issue in evaluating biological processes. The current clinical practice involves the application of a tedious and time consuming manual tracking procedure on large amount of data. As a result, automatic tracking systems are currently developed and evaluated. However, problems caused by cellular division, agglomeration, Brownian motion and topology changes are difficult issues that have to be accommodated by automatic tracking techniques. In this paper, we detail the development of a fully automated multi-target tracking system that is able to deal with Brownian motion and cellular division. During the tracking process our approach includes the neighbourhood relationship and motion history to enforce the cellular tracking continuity in the spatial and temporal domain. The experimental results reported in this paper indicate that our method is able to accurately track cellular structures in time-lapse data.

Item Type:Conference or Workshop Item (Paper)
Event Type:Conference
Refereed:Yes
Uncontrolled Keywords:Brownian motion; biological techniques; cellular biophysics; feature extraction; object detection; tracking;
Subjects:Computer Science > Image processing
DCU Faculties and Centres:Research Initiatives and Centres > Research Institute for Networks and Communications Engineering (RINCE)
Published in:Proceedings of the 13th International Machine Vision and Image Processing Conference. . Institute of Electrical and Electronics Engineers. ISBN 978-1-4244-4875-3
Publisher:Institute of Electrical and Electronics Engineers
Official URL:http://dx.doi.org/10.1109/IMVIP.2009.21
Copyright Information:©2009 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE.
Funders:Higher Education Authority
ID Code:15571
Deposited On:27 Jul 2010 13:31 by DORAS Administrator. Last Modified 27 Jul 2010 13:31

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