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Effect of substrate thermal resistance on space-domain microchannel

Kinahan, David J. and Dalton, Tara M. and Davies, Mark RD. (2009) Effect of substrate thermal resistance on space-domain microchannel. Biomedical Microdevices, 11 (4). pp. 747-754. ISSN 1387-2176

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Abstract

In recent years, Fluorescent Melting Curve Analysis (FMCA) has become an almost ubiquitous feature of commercial quantitative PCR (qPCR) thermal cyclers. Here a micro-fluidic device is presented capable of performing FMCA within a microchannel. The device consists of modular thermally conductive blocks which can sandwich a microfluidic substrate. Opposing ends of the blocks are held at differing temperatures and a linear thermal gradient is generated along the microfluidic channel. Fluorescent measurements taken from a sample as it passes along the micro-fluidic channel permits fluorescent melting curves to be generated. In this study we measure DNA melting temperature from two plasmid fragments. The effects of flow velocity and ramp-rate are investigated, and measured melting curves are compared to those acquired from a commercially available PCR thermocycler.

Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:PCR; μTAS; DNA denaturation; Fluorescent melting curve analysis; Microchannel; Thermal resistance
Subjects:Engineering > Biomedical engineering
DCU Faculties and Centres:UNSPECIFIED
Publisher:Kluwer (Springer)
Official URL:http://dx.doi.org/10.1007/s10544-009-9288-6
Copyright Information:© 2009 Springer The original publication is available at www.springerlink.com
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
ID Code:17160
Deposited On:15 Aug 2012 11:14 by David Kinahan. Last Modified 15 Aug 2012 11:14

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