Optimisation of the enzyme linked lectin assay (ELLA) for enhanced glycoprotein and glycoconjugate analysis
Thompson, Roisin and Creavin, Aileen and O'Connell, Michael and Clarke, Paul A. and O'Connor, Brendan (2011) Optimisation of the enzyme linked lectin assay (ELLA) for enhanced glycoprotein and glycoconjugate analysis. In: Eurocarb 16, 3-7 Jul 2011, Sorrento, Italy.
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The ability of lectins to recognise and bind specific oligosaccharide structures has been exploited frequently in analytical platforms. However, a major difficulty encountered in lectin-based analysis, particularly in multi- well plate formats, such as Enzyme-Linked Lectin Assays (ELLA’s), is a lack of appropriate blocking solutions to prevent non-specific interactions during the course of an experiment. We have attempted to address this issue by assessing a range of blocking reagents using a panel of 19 biotinylated lectins exhibiting diverse structures and carbohydrate specificities.
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