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Optimisation of the enzyme linked lectin assay (ELLA) for enhanced glycoprotein and glycoconjugate analysis

Thompson, Roisin and Creavin, Aileen and O'Connell, Michael and Clarke, Paul A. and O'Connor, Brendan (2011) Optimisation of the enzyme linked lectin assay (ELLA) for enhanced glycoprotein and glycoconjugate analysis. In: Eurocarb 16, 3-7 Jul 2011, Sorrento, Italy.

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Abstract

The ability of lectins to recognise and bind specific oligosaccharide structures has been exploited frequently in analytical platforms. However, a major difficulty encountered in lectin-based analysis, particularly in multi- well plate formats, such as Enzyme-Linked Lectin Assays (ELLA’s), is a lack of appropriate blocking solutions to prevent non-specific interactions during the course of an experiment. We have attempted to address this issue by assessing a range of blocking reagents using a panel of 19 biotinylated lectins exhibiting diverse structures and carbohydrate specificities.

Item Type:Conference or Workshop Item (Poster)
Event Type:Conference
Refereed:Yes
Uncontrolled Keywords:lectins; analytical platforms
Subjects:Biological Sciences > Biotechnology
Biological Sciences > Biochemistry
Biological Sciences > Biosensors
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology
Research Initiatives and Centres > Irish Separation Science Cluster (ISSC)
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
Funders:Science Foundation Ireland
ID Code:17759
Deposited On:27 Feb 2013 14:03 by Brendan O'Connor. Last Modified 15 Feb 2017 16:58

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