Gel filtration chromatography
Ó Fágáin, Ciarán and Cummins, Phil and O'Connor, Brendan (2011) Gel filtration chromatography. In: Walls, Dermot and Loughran, Sinéad T., (eds.) Protein Chromatography. Methods in Molecular Biology, 681 . Humana Publishers Ltd., pp. 25-35. ISBN 978-1-60761-913-0
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Gel-filtration chromatography is a popular and versatile technique that permits the effective separation of proteins and other biological molecules in high yield. Here, the basis of the method is described and typical matrix types are contrasted. The selection of suitable operating conditions and applications of the method are also discussed.
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