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Gel filtration chromatography

Ó Fágáin, Ciarán and Cummins, Phil and O'Connor, Brendan (2011) Gel filtration chromatography. In: Walls, Dermot and Loughran, Sinéad T., (eds.) Protein Chromatography. Methods in Molecular Biology, 681 . Humana Publishers Ltd., pp. 25-35. ISBN 978-1-60761-913-0

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Abstract

Gel-filtration chromatography is a popular and versatile technique that permits the effective separation of proteins and other biological molecules in high yield. Here, the basis of the method is described and typical matrix types are contrasted. The selection of suitable operating conditions and applications of the method are also discussed.

Item Type:Book Section
Refereed:Yes
Uncontrolled Keywords:Chromatographic methods; Microfluidics; Native protein isolation; Protein biochemistry; Protein quantitation; Purification schemes; Recombinant antibody production
Subjects:Biological Sciences > Biotechnology
Biological Sciences > Biochemistry
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology
Research Initiatives and Centres > Irish Separation Science Cluster (ISSC)
Publisher:Humana Publishers Ltd.
Official URL:http://www.springer.com/new+%26+forthcoming+titles+(default)/book/978-1-60761-912-3
Copyright Information:© 2011 Springer (Humana)
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
Funders:Science Foundation Ireland, Enterprise Ireland
ID Code:17808
Deposited On:19 Feb 2013 11:20 by Brendan O'Connor. Last Modified 13 Feb 2017 11:45

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