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Polymerisation and surface modification of methacrylate monoliths in polyimide channels and polyimide coated capillaries using 660 nm light emitting diodes

Walsh, Zarah and Levkin, Paul A. and Abele, Silvija and Scarmagnani, Silvia and Heger, Dominik and Klán, Petr and Diamond, Dermot and Paull, Brett and Svec, Frantisek and Macka, Mirek (2011) Polymerisation and surface modification of methacrylate monoliths in polyimide channels and polyimide coated capillaries using 660 nm light emitting diodes. Journal of Chromatography A, 1218 (20). pp. 2954-2962. ISSN 0021-9673

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Abstract

An investigation into the preparation of monolithic separation media utilising a cyanine dye sensitiser/triphenylbutylborate/N-methoxy-4-phenylpyridinium tetrafluoroborate initiating system activated by 660nm light emitting diodes is reported. The work demonstrates multiple uses of red- light initiated polymerisation in the preparation of monolithic stationary phases within polyimide and polyimide coated channels and the modification of monolithic materials with molecules which absorb strongly in the UV region. This initiator complex was used to synthesise poly(butyl methacrylate- co-ethylene dimethacrylate) and poly(methyl methacrylate-co-ethylene dimethacrylate) monolithic stationary phases in polyimide coated fused silica capillaries of varying internal diameters, as well as within polyimide micro-fluidic chips. The repeatability of the preparation procedure and resultant mono- lithic structure was demonstrated with a batch of poly(butyl methacrylate-co-ethylene dimethacrylate) monoliths in 100 􏰀m i.d. polyimide coated fused silica capillary, which were applied to the separation of a model protein mixture (ribonuclease A, cytochrome C, myoglobin and ovalbumin). Taking an average from 12 chromatograms originating from each batch, the maximum relative standard deviation of the retention factor (k) for the protein separations was recorded as 0.53%, the maximum variance for the selectivity factor ( ̨) was 0.40% while the maximum relative standard deviation in peak resolution was 8.72%. All maxima were recorded for the Ribonuclease A/Cytochrome C peaks. Scanning electron microscopy confirmed the success of experiments in which poly(butyl methacrylate- co-ethylene dimethacrylate) monoliths were prepared using the same initiation approach in capillary and micro-fluidic chips, respectively. The initiating system was also applied to the photo-initiated grafting of a chromophoric monomer onto poly(butyl methacrylate-co-ethylene dimethacrylate) monoliths within poly(tetrafluoroethylene) coated fused silica capillaries.

Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:Photo-initiated polymerisation; Micro-fluidic chips
Subjects:Physical Sciences > Analytical chemistry
Engineering > Materials
DCU Faculties and Centres:Research Initiatives and Centres > Irish Separation Science Cluster (ISSC)
Research Initiatives and Centres > National Centre for Sensor Research (NCSR)
Research Initiatives and Centres > CLARITY: The Centre for Sensor Web Technologies
Publisher:Elsevier
Official URL:http://dx.doi.org/10.1016/j.chroma.2011.03.021,
Copyright Information:© 2011 Elsevier
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
Funders:Marie Curie Excel- lence Grants and Funding (MEXT-CT-2004-014361), Science Foundation Ireland under the Irish Separation Science Cluster award (Grant Number 08/SRC/B1412)., Science Foundation Ireland under the Science Foundation Ireland Research Frontiers Programme (SFI 03/IN.3/1361; 07/RPF/MASF812 and 07/CE/I1147), Ministry of Education, Youth and Sports of the Czech Republic (MSM0021622413) , European Union (CETOCOEN, CZ.1.05/2.1.00/01.0001), National Institute of Biomedical Imag- ing and Bioengineering, National Institutes of Health (EB-006133), Office of Science, Office of Basic Energy Sciences, U.S. Department of Energy, under Contract No. DEAC02-05CH11231
ID Code:17899
Deposited On:09 Apr 2013 10:39 by Kevin Fraser. Last Modified 09 Nov 2016 14:31

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