Browse DORAS
Browse Theses
Search
Latest Additions
Creative Commons License
Except where otherwise noted, content on this site is licensed for use under a:

Probing the specific ion effects of biocompatible hydrated choline ionic liquids on lactate oxidase biofunctionality in sensor applications

Curto, Vincenzo F. and Scheuermann, Stefan and Owens, Roisin and Ranganathan, Vijayaraghavan and MacFarlane, Douglas R. and Benito-Lopez, Fernando and Diamond, Dermot (2013) Probing the specific ion effects of biocompatible hydrated choline ionic liquids on lactate oxidase biofunctionality in sensor applications. Physical Chemistry Chemical, 2014 (16). pp. 1841-1849. ISSN 1463-9076

Full text available as:

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
1550Kb

Abstract

This paper presents an extended study on the ion effects of a series of biocompatible hydrated choline based ionic liquids (ILs) on lactate oxidase (LOx), an important enzyme in biosensing technology for the in vitro detection of lactic acid. Secondary structural analysis revealed changes in the protein conformation in hydrated ILs, while thermal unfolding/aggregation dynamics showed different profiles in the presence or absence of ILs. Moreover, LOx thermally denaturised at 90 1C showed residual activity in the presence of chloride and dihydrogen phosphate anions. Kinetic and lifetime studies were also performed, providing a better understanding of the ion effects of ILs on the biocatalytic activity of the enzyme.

Item Type:Article (Published)
Refereed:Yes
Subjects:Physical Sciences > Analytical chemistry
Engineering > Materials
Biological Sciences > Biosensors
DCU Faculties and Centres:Research Initiatives and Centres > INSIGHT Centre for Data Analytics
Research Initiatives and Centres > National Centre for Sensor Research (NCSR)
Publisher:Royal Society of Chemistry
Official URL:http://dx.oid.org/10.1039/c3cp52845f
Copyright Information:© 2013 RSC
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
Funders:VFC, SS, FBL, and DD acknowledge Science Foundation Ireland (grant 07/CE/I1147) , SS is grateful to the Erasmus placement programme of the European Lifelong Learning Programme (LLP), RMO acknowledges funding from the European Research Council (ERC grant agreement no. 258966)., MASK, a Marie Curie International Research Staff Exchange Scheme
ID Code:19709
Deposited On:28 Jan 2014 14:26 by Kevin Fraser. Last Modified 27 Apr 2017 15:34

Download statistics

Archive Staff Only: edit this record