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Lipophilic-membrane based routing for centrifugal automation of heterogeneous immunoassays

Mishra, Rohit ORCID: 0000-0003-4279-4163, Alam, Rizwan, Kinahan, David J. ORCID: 0000-0003-1968-2016, Anderson, Karen and Ducrée, Jens ORCID: 0000-0002-0366-1897 (2015) Lipophilic-membrane based routing for centrifugal automation of heterogeneous immunoassays. In: 28th International Conference on Micro Electro Mechanical Systems (MEMS), 2015 IEEE, 18-22 Jan 2015, Estoril, Portugal. ISBN 978-1-4799-7955-4

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Abstract

We demonstrate centrifugal [liquid handling] automation of an Enzyme-Linked Immuno-Sorbent Assay (ELISA) for the detection of anti-p53 antibodies in whole blood. On this “Lab-on-a-Disc” (LoaD) platform, all unit operations were implemented by event-triggered rotational flow control. In order to avoid interference during absorbance measurement from the solid phase in this heterogeneous assay format, it is pivotal that the intermediate reaction product is eventually forwarded from the incubation chamber to a distinct optical measurement chamber. To this end we have devised routing of flows by lipophilic film valves (LFVs) which remain intact in aqueous and selectively dissolve when exposed to an ancillary, oleophilic solvent.

Item Type:Conference or Workshop Item (Poster)
Event Type:Conference
Refereed:Yes
Uncontrolled Keywords:ELISA; Centrifugal microfluidics
Subjects:Engineering > Materials
Biological Sciences > Microfluidics
DCU Faculties and Centres:UNSPECIFIED
Published in: Proceedings of 28th International Conference on Micro Electro Mechanical Systems (MEMS). . IEEE. ISBN 978-1-4799-7955-4
Publisher:IEEE
Copyright Information:© 2015 IEEE
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
ID Code:20442
Deposited On:11 Feb 2015 11:05 by Rohit Mishra . Last Modified 26 Oct 2018 09:58

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