Stokes Bio, founded in 2005, develops innovative microfluidic technologies. In 2008 in collaboration with Monsanto, an application driven development for a high-throughput instrument in the detection and characterisation of Single Nucleotide Polymorphisms (SNPs) in agricultural crops was initiated.
Stokes technology is designed to generate aqueous nanolitre scale droplets of reagents and
samples, wrapped in a carrier fluid from standard microtitre plates and to mix them using Stokes
Bio’s proprietary liquid bridge mixers. Following mixing the complete assay is transferred in the
carrier fluid through the use of a continuous flow system, to a flow through thermal cycler and an
optical reading station.
This poster summarises results collated using the Stokes Bio genotyping platform currently based in Monsanto. Data will be presented to illustrate the dynamic capabilities of the instrument, highlighting the enhanced sensitivity and reproducibility of PCR in droplet format compared to well-based technologies.