Solvent-selective membranes for automating sequential liquid release and routing of nucleic acid purification protocols on a simple spindle motor
Gaughran, JenniferORCID: 0000-0002-3659-036X, Kinahan, David J.ORCID: 0000-0003-1968-2016, Mishra, RohitORCID: 0000-0003-4279-4163 and Ducrée, JensORCID: 0000-0002-0366-1897
(2016)
Solvent-selective membranes for automating sequential liquid release and routing of nucleic acid purification protocols on a simple spindle motor.
In: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 9-13 Oct 2016, Dublin, Ireland.
By incorporating a set of membranes which selectively dissolve upon contact with aqueous or or-ganic solvents at strategic locations on a disc cartridge, we succeeded to fully automate the solid-phase extraction of nucleic acids by varying the spin rate of a low-cost spindle motor. A solvent- and phase-selective graphene oxide (GO) membrane governs the routing of flows to designated elution and waste chambers. The serial release of on-board sample and reagents is centrifugo-pneumatically controlled by our previously introduced, event-triggered valving scheme. The entire process of ex-traction completes in less than 8 minutes.