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Amperometric separation-free immunosensor for real-time environmental monitoring

Killard, Anthony J. and Micheli, Laura and Grennan, Kathleen and Franek, Milan and Kolar, Vladimir and Moscone, Danila and Palchetti, Ilaria and Smyth, Malcolm R. (2000) Amperometric separation-free immunosensor for real-time environmental monitoring. Analytica Chimica Acta, 427 (2). pp. 173-180. ISSN 0003-2670

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Abstract

Immunoanalytical techniques have found widespread use due to the characteristics of specificity and wide applicability for many analytes, from large polymer antigens, to simple haptens, and even single atoms. Electrochemical sensors offer benefits of technical simplicity, speed and convenience via direct transduction to electronic equipment. Together, these two systems offer the possibility of a convenient, ubiquitous assay technique with high selectivity. However, they are still not widely used, mainly due to the complexity of the associated immunoassay methodologies. A separation-free immunoanalytical technique is described here, which has allowed for the analysis of atrazine in real time and in both quasi-equilibrium and stirred batch configurations. It illustrated that determinations as low as 0.13mM (28 ppb) could be made using equilibrium incubation with an analytical range of 0.1–10mM. Measurements could be made between 1 and 10 mM within several minutes using a real-time, stirred batch method. This system offers the potential for fast, simple, cost-effective biosensors for the analysis of many substances of environmental, biomedical and pharmaceutical concern.

Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:immunosensor; atrazine; polyaniline; real-time;
Subjects:Physical Sciences > Electrochemistry
Physical Sciences > Chemistry
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Chemical Sciences
Research Initiatives and Centres > National Centre for Plasma Science and Technology (NCPST)
Research Initiatives and Centres > National Centre for Sensor Research (NCSR)
Publisher:Elsevier
Official URL:http://dx.doi.org/10.1016/S0003-2670(00)01015-1
ID Code:23
Deposited On:08 Nov 2006 by DORAS Administrator. Last Modified 30 Jan 2009 11:10

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