Mediated amperometric immunosensing using single walled
carbon nanotube forests
O'Connor, Máire B., Kim, Sang Nyon, Killard, Anthony J.ORCID: 0000-0001-6953-3655, Forster, Robert J., Smyth, Malcolm R., Papadimitrakopoulos, Fotios and Rusling, James F.
(2004)
Mediated amperometric immunosensing using single walled
carbon nanotube forests.
Analyst, 129
(12).
pp. 1176-1180.
ISSN 0003-2654
A prototype amperometric immunosensor was evaluated based on the adsorption of antibodies onto perpendicularly oriented assemblies of single wall carbon nanotubes called SWNT forests. The forests were self-assembled from oxidatively shortened SWNTs onto Nafion/iron oxide coated pyrolytic graphite electrodes. The nanotube forests were characterized using atomic force microscopy and resonance Raman spectroscopy. Anti-biotin antibody strongly adsorbed to the SWNT forests. In the presence of a soluble mediator, the detection limit for horseradish peroxidase (HRP) labeled biotin was 2.5 pmol ml[-1] (2.5 nM). Unlabelled biotin was detected in a competitive approach with a detection limit of 16 nmol ml[-1] (16 μM) and a relative standard deviation of 12%. The immunosensor showed low non-specific adsorption of biotin-HRP (approx. 0.1%) when blocked with bovine serum albumin. This immunosensing approach using high surface area, patternable, conductive SWNT assemblies may eventually prove useful for nano-biosensing arrays.
Metadata
Item Type:
Article (Published)
Refereed:
Yes
Uncontrolled Keywords:
enzyme; oxidoreductases; peroxidases; serum albumin; peroxidase; biotin; graphite; iron oxide; antibody; chemical enrichment; chemical sensor; biosensor; surface area; standard deviation; detection limit; raman spectrometry; atomic force microscopy; adsorption; prototype; carbon nanotubes; immunosensor; amperometry;