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An amperometric enzyme biosensor fabricated from polyaniline nanoparticles

Morrin, Aoife and Ngamna, Orawan and Killard, Anthony J. and Moulton, Simon E. and Smyth, Malcolm R. and Wallace, Gordon G. (2005) An amperometric enzyme biosensor fabricated from polyaniline nanoparticles. Electroanalysis, 17 (5-6). pp. 423-430. ISSN 1040-0397

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Abstract

The engineered addition of hexa-histidine sequences to biomolecules such as antibody fragments has been found to be an excellent means of purifying these materials. This tagging methodology has also been extended to its use as a tool for immobilization and orientation of antibodies on transducer surfaces. Polyvinyl sulfonate-doped polyanilne (PANI/PVS) can be used as a mediator in amperometric biosensors. This short communication looks at the effect of nickel chelate materials and nickel chelation on this conducting polymer and evaluates it as a potential surface for the immobilization of his-tagged biomolecules. N-nitrilotriacetic acid (NTA) was doped into the electropolymerized PANI/PVS at a screen-printed carbon paste electrode. The resulting NTA-PANI/PVS film was shown to have comparable electrochemical properties of polymer without the chelating agent. When Ni2+ was applied to the electrode, the incorporated NTA was found to efficiently chelate the metal ions at the electrode surface.

Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:amperometric biosensor; protein immobilization; histidine tag; nitrilotriacetic acid; nickel; orientation;
Subjects:Physical Sciences > Chemistry
Biological Sciences > Biosensors
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Chemical Sciences
Research Initiatives and Centres > National Centre for Sensor Research (NCSR)
Publisher:John Wiley & Sons
Official URL:http://dx.doi.org/10.1002/elan.20040318
ID Code:52
Deposited On:08 Nov 2006 by DORAS Administrator. Last Modified 30 Jan 2009 11:57

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