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Optimisation and characterisation of biosensors based on polyaniline

Grennan, Kathleen and Killard, Anthony J. and Hanson, Claire J. and Cafolla, Attilio A. and Smyth, Malcolm R. (2005) Optimisation and characterisation of biosensors based on polyaniline. Talanta, 68 (5). pp. 1591-1600. ISSN 0039-9140

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Abstract

With lower limits of detection and increased stability constantly being demanded of biosensor devices, characterisation of the constituent layers that make up the sensor has become unavoidable, since this is inextricably linked with its performance. This work describe the optimisation and characterisation of two aspects of sensor performance: a conductive polymer layer (polyaniline) and the immobilised protein layer. The influence of the thickness of polyaniline films deposited electrochemically onto screen-printed electrode surfaces is described in this work in terms of its influence on a variety of amperometric sensor performance characteristics: time to reach steady state, charging current, catalytic current, background current and signal/background ratios. The influence of polymer film thickness on the conductivity and morphology of finished films is also presented. An electrostatic method of protein immobilisation is used in this work and scanning electron microscopy in conjunction with gold-labelled antibodies and back-scattered electron detection has enabled the direct visualisation of individual groups of proteins on the sensor surface. Such information can provide an insight into the performance of sensors under influence of increasing protein concentrations.

Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:characterisation; biosensor; polyaniline; screen-printed electrodes; scanning electron microscopy; amperometry; atomic force microscopy;
Subjects:Physical Sciences > Electrochemistry
Physical Sciences > Chemistry
Biological Sciences > Biosensors
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Chemical Sciences
Research Initiatives and Centres > National Centre for Sensor Research (NCSR)
DCU Faculties and Schools > Faculty of Science and Health > School of Physical Sciences
Publisher:Elsevier
Official URL:http://dx.doi.org/10.1016/j.talanta.2005.08.036
ID Code:57
Deposited On:08 Nov 2006 by DORAS Administrator. Last Modified 30 Jan 2009 12:08

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