Direct hemoglobin measurement by monolithically integrated optical beam guidance
Grumann, M., Dube, M., Brefka, T., Steigert, J., Riegger, L., Brenner, T., Mittrnann, K., Zengerle, Roland and Ducrée, JensORCID: 0000-0002-0366-1897
(2005)
Direct hemoglobin measurement by monolithically integrated optical beam guidance.
In: TRANSDUCERS 2005 - 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 5-9 June 2005, Seoul, Korea.
ISBN 0-7803-8994-8
We present a concept for optical beam guidance by total internal reflection (TIR) at V-grooves as retro reflectors which are monolithically integrated on a microfluidic "lab-on-a-disk". This way, the optical path length through a measurement chamber and thus the sensitivity of colorimetric assays is massively enhanced compared to direct (perpendicular) beam incidence. With this rugged optical concept, we determine the concentration of hemoglobin (Hb) in human whole blood. Outstanding features are a high degree of linearity (R2 = 0.993) between the optical signal and the Hb together with a reproducibility of CV= 2.9 %, and a time-to-result of 100 seconds, only.
TRANSDUCERS 2005 - 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers.
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Institute of Electrical and Electronics Engineers. ISBN 0-7803-8994-8