Parallelization of chip-based fluorescence immuno-assays with quantum-dot labelled beads
Grumann, M., Riegger, L., Nann, T., Riegler, J., Ehlert, O., Mittenbuhler, K., Urban, G., Pastewka, L., Brenner, T., Zengerle, Roland and Ducrée, JensORCID: 0000-0002-0366-1897
(2005)
Parallelization of chip-based fluorescence immuno-assays with quantum-dot labelled beads.
In: TRANSDUCERS 2005 - 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 5-9 June 2005, Seoul, Korea.
ISBN 0-7803-8994-8
This paper presents an optical concept for the read-out of a parallel, bead-based fluorescence immunoassay conducted on a lab-on-a-disk platform. The reusable part of the modular setup comprises a detection unit featuring a single LED as light source, two emission-filters, and a color CCD-camera as standard components together with a spinning drive as actuation unit. The miniaturized lab-on-a-disk is devised as a disposable. In the read-out process of the parallel assay, beads are first identified by the color of incorporated quantum dots (QDs). Next, the reaction-specific fluorescence signal is quantified with FluoSpheres-labeled detection anti-bodies. To enable a fast and automated read-out, suitable algorithms have been implemented in this work. Based on this concept, we successfully demonstrated a Hepatitis-A assay on our disk-based lab-on-a-chip.
TRANSDUCERS 2005 - 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers.
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Institute of Electrical and Electronics Engineers. ISBN 0-7803-8994-8