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Optimisation of the enzyme linked lectin assay (ELLA) for enhanced glycoprotein and glycoconjugate analysis

Thompson, Roisin, Creavin, Aileen, O'Connell, Michael, Clarke, Paul A. and O'Connor, Brendan orcid logoORCID: 0000-0002-8999-5184 (2011) Optimisation of the enzyme linked lectin assay (ELLA) for enhanced glycoprotein and glycoconjugate analysis. In: Eurocarb 16, 3-7 Jul 2011, Sorrento, Italy.

The ability of lectins to recognise and bind specific oligosaccharide structures has been exploited frequently in analytical platforms. However, a major difficulty encountered in lectin-based analysis, particularly in multi- well plate formats, such as Enzyme-Linked Lectin Assays (ELLA’s), is a lack of appropriate blocking solutions to prevent non-specific interactions during the course of an experiment. We have attempted to address this issue by assessing a range of blocking reagents using a panel of 19 biotinylated lectins exhibiting diverse structures and carbohydrate specificities.
Item Type:Conference or Workshop Item (Poster)
Event Type:Conference
Uncontrolled Keywords:lectins; analytical platforms
Subjects:Biological Sciences > Biotechnology
Biological Sciences > Biochemistry
Biological Sciences > Biosensors
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology
Research Institutes and Centres > Irish Separation Science Cluster (ISSC)
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
Funders:Science Foundation Ireland
ID Code:17759
Deposited On:27 Feb 2013 14:03 by Brendan O'connor . Last Modified 03 Oct 2018 14:58

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