Production of lectin-affinity matrices for process-scale glycoprotein purification
Thompson, Roisin, Keogh, Damien, O'Connell, Michael, Clarke, Paul A. and O'Connor, BrendanORCID: 0000-0002-8999-5184
(2011)
Production of lectin-affinity matrices for process-scale glycoprotein purification.
In: Research Review Day 2011, 15 Jun 2011, DCU, Dublin, Ireland.
A selection of prokaryotic lectins with a variety of glycan specificities and affinities have been identified, cloned, expressed in Eschericia coli and characterised. The aims of this project are to:
- express the lectins at 1L scale to produce sufficient quantities for immobilisation studies (~100 mg)
- immobilisethelectinsonSepharose
- evaluate lectin performance on column by monitoring their ability toreproducibly capture and elute glycoprotein glycoforms.