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Siderophore mediated iron acquisation by Sinorhizobium meliloti.

Cooke, Colm (2014) Siderophore mediated iron acquisation by Sinorhizobium meliloti. PhD thesis, Dublin City University.

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Abstract

Sinorhizobium meliloti 2011 is a free living soil bacterium notable for being the intracellular nitrogen fixing symbiont of Medicago sativa. Like most bacteria iron is vital to its survival in both symbiotic and free living conditions. It produces an asymmetrically lipidated siderophore, rhizobactin 1021 (rhz1021), in response to iron limitation. Previous studies have attributed rhz1021 production to the gene products of the operon rhbABCDEF. The roles of two acetyltransferases in rhz1021 biosynthesis, rhbD and an unstudied gene sma2339 have been assessed. Analysis shows that S. meliloti 2011sma2339 is capable of producing an intact rhz1021 structure but at a reduced rate to wild type and that the sma2339 gene can poorly complement rhbD. The rhbD mutant strain is highly defective in siderophore production confirming its central role in biosynthesis.

Item Type:Thesis (PhD)
Date of Award:November 2014
Refereed:No
Supervisor(s):O'Connell, Michael
Subjects:Biological Sciences > Genetics
Biological Sciences > Microbiology
Biological Sciences > Molecular biology
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology
Research Initiatives and Centres > Irish Separation Science Cluster (ISSC)
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 License. View License
Funders:Science Foundation Ireland
ID Code:20139
Deposited On:21 Nov 2014 14:40 by Brendan O'connor . Last Modified 19 Jul 2018 15:04

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