Buoyancy-driven centripetal pumping for nested sample preparation in bioassays
Kilcawley, NiamhORCID: 0000-0003-1968-2016, Kinahan, David J., Nwankire, Charles, Glynn, MacdaraORCID: 0000-0002-6347-4348 and Ducrée, JensORCID: 0000-0002-0366-1897
(2014)
Buoyancy-driven centripetal pumping for nested sample preparation in bioassays.
In: The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2014), 26-30 Oct 2014, San Antonio, TX..
In this paper we present a method of buoyancy-driven centripetal pumping utilizing a heavy, water-immiscible liquid to displace an aqueous sample radially inwards against the prevailing centrifugal force. Using formerly introduced dissolvable-film valving, we demonstrate metering and bidirectional periodical pumping of a single sample in the radial direction. We then show integrated sample discretisation, meter-ing and buoyancy-driven inward pumping in a microfluidic structure designed towards multi-parameter bioassays. This structure permits centrifugation of sample on the periphery of a disc (where the centrifugal field p) and proves a high degree of metering accuracy better than 1.2%