Rotational-pulse actuated dissolvable-film valves for automated purification of total RNA from E. Coli
Kinahan, David J.ORCID: 0000-0003-1968-2016, Clancy, Eoin, Kilcawley, Niamh, Dimov, Nikolay, Smith, Terry and Ducrée, JensORCID: 0000-0002-0366-1897
(2014)
Rotational-pulse actuated dissolvable-film valves for automated purification of total RNA from E. Coli.
In: The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2014), 26-30 Oct 2014, San Antonio, TX..
In this work we report for the first time on a repertoire of valving technologies which are combined to enable automated purification of total RNA from cell homogenate. Process control is implemented us-ing rotational-pulse actuated dissolvable-film (DF) valves; where the order of valve actuation is deter-mined by the disc architecture while the timing of valve actuation is governed by pulses in the spin rate. Selective liquid routing is enabled by combining a heavy, inert and immiscible liquid plug with a DF. The combination of these technologies enables bead-based extraction of amplifiable RNA, with a yield comparable to gold-standard bench-top protocols.