Lipophilic-membrane based routing for centrifugal automation of heterogeneous immunoassays
Mishra, RohitORCID: 0000-0003-4279-4163, Alam, Rizwan, Kinahan, David J.ORCID: 0000-0003-1968-2016, Anderson, Karen and Ducrée, JensORCID: 0000-0002-0366-1897
(2015)
Lipophilic-membrane based routing for centrifugal automation of heterogeneous immunoassays.
In: 28th International Conference on Micro Electro Mechanical Systems (MEMS), 2015 IEEE, 18-22 Jan 2015, Estoril, Portugal.
ISBN 978-1-4799-7955-4
We demonstrate centrifugal [liquid handling]
automation of an Enzyme-Linked Immuno-Sorbent Assay
(ELISA) for the detection of anti-p53 antibodies in whole
blood. On this “Lab-on-a-Disc” (LoaD) platform, all unit
operations were implemented by event-triggered rotational
flow control. In order to avoid interference during
absorbance measurement from the solid phase in this
heterogeneous assay format, it is pivotal that the intermediate reaction product is eventually forwarded from the incubation chamber to a distinct optical measurement
chamber. To this end we have devised routing of flows by
lipophilic film valves (LFVs) which remain intact in
aqueous and selectively dissolve when exposed to an
ancillary, oleophilic solvent.