Staging the clinical status from blood of cancer patients by chip-based cell enumeration following targeted removal of normal cells
Glynn, MacdaraORCID: 0000-0002-6347-4348, Kirby, Daniel, Nwankire, Charles, Kinahan, David J.ORCID: 0000-0003-1968-2016, Spillane, Cathy, Shiels, Orla, O'Leary, John and Ducrée, JensORCID: 0000-0002-0366-1897
(2014)
Staging the clinical status from blood of cancer patients by chip-based cell enumeration following targeted removal of normal cells.
In: The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014), 26-30 Oct 2014, San Antonio, TX..
Even though an agreed phenotypic definition of circulating tumor cells (CTCs) remains elusive in the literature, many current detection technologies isolate candidate cells based on molecular recognition of cellular epitopes that may not accurately predict CTC load. Rather than using such an epitope specific “positive-capture” strategy, we present a chip-based, centrifugal microfluidic platform integrating “negative-capture” magnetophoretic removal of normal white blood cells (WBCs) from a sample and subsequent, array-based enumeration of individualized, (untagged) abnormal cells. We compared the numerical recovery of cells on the array with the status of the donor patient, showing that the chip can has the potential to indicate the oncogenic severity of the blood donor.
Metadata
Item Type:
Conference or Workshop Item (Poster)
Event Type:
Conference
Refereed:
Yes
Uncontrolled Keywords:
Lab-on-a-Disc; Magnetic Selection; Magnetophoresis; CTC detection