Automation of heterogeneous prostate cancer immunoassay based on multi-valve triggering using purely rotational flow control
Mishra, RohitORCID: 0000-0003-4279-4163, Zapatero-Rodríguez, Julia, Sharma, Shikha, Kelly, Domhnall, McAuley, Darren, O'Kennedy, Richard and Ducrée, JensORCID: 0000-0002-0366-1897
(2016)
Automation of heterogeneous prostate cancer immunoassay based on multi-valve triggering using purely rotational flow control.
In: The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 9-13 Oct. 2016, Dublin, Ireland.
A multi-marker approach is increasingly considered in diagnosis of prostate cancer to minimize the number of unnecessary biopsies [1]. Centrifugo-pneumatic flow control is often the method of choice for multiplexing such assays [2]. However, the number of assays that can be parallelized on such a dissolvable-film (DF) based system is limited by the substantial spread of the burst frequencies [2] owing to manufacturing tolerances. In this work we substantially enhance the definition of actuation frequencies by 75% using a novel design to enable the automation of multi-step / multi-reagent Lab-on-a-Disc (LoaD) platform. We demonstrate a multi-marker prostate cancer immunoassay panel.
Item Type:
Conference or Workshop Item (Poster)
Event Type:
Conference
Refereed:
Yes
Uncontrolled Keywords:
Immunoassays; Centrifugal microfluidics; dissolvable film valving; prostate cancer
This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
Funders:
European Commission Grant no. 317420, 2012-2016); Science Foundation Ireland (SFI) under Grant No. 10/CE/B1821; SFI-TIDA 13/TIDA/B2692 and SFI-TIDA 14/TIDA/2264.
ID Code:
21453
Deposited On:
26 Oct 2016 09:52 by
Rohit Mishra
. Last Modified 27 Aug 2018 09:35