Automated DNA purification and Multiplexed lamp assay preparation On a centrifugal microfluidic “lab-on-a-disc” platform
Kinahan, David J.ORCID: 0000-0003-1968-2016, Julius, Lourdes A.N., Schoen, Cor, Dreo, Tanja and Ducrée, JensORCID: 0000-0002-0366-1897
(2018)
Automated DNA purification and Multiplexed lamp assay preparation On a centrifugal microfluidic “lab-on-a-disc” platform.
In: The 31st IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2018), 21-25 Jan, 2018, Belfast, Northern Ireland, UK,.
ISBN ESSN: 2160-1968
This work presents a rotational-pulse actuated microfluidic cartridge enabling automated detection of plant pathogens on a compact device towards point-of-use monitoring of food safety. This highly integrated “Lab-on-a-Disc” (LoaD) system first runs the sample over a stationary phase of silica beads, followed by ethanol (EtOH) wash and final elution of DNA. The eluate is then homogenized using ‘shake mode’ agitation, accurately metered and then mixed with reagents for loop-mediated isothermal amplification (LAMP). We successfully purify plant DNA and demonstrate on-disc quantitative LAMP amplification.
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Funders:
European Union (FP7-KBBE-2013-7-613908-DECATHLON, Science Foundation Ireland (SFI) and Fraunhofer Gesellschaft under its Strategic Partnership Programme Grant No.r 16/SPP/3321.
ID Code:
22228
Deposited On:
26 Jan 2018 14:41 by
David Kinahan
. Last Modified 30 Jul 2019 10:28