Login (DCU Staff Only)
Login (DCU Staff Only)

DORAS | DCU Research Repository

Explore open access research and scholarly works from DCU

Advanced Search

Automated DNA purification and Multiplexed lamp assay preparation On a centrifugal microfluidic “lab-on-a-disc” platform

Kinahan, David J. orcid logoORCID: 0000-0003-1968-2016, Julius, Lourdes A.N., Schoen, Cor, Dreo, Tanja and Ducrée, Jens orcid logoORCID: 0000-0002-0366-1897 (2018) Automated DNA purification and Multiplexed lamp assay preparation On a centrifugal microfluidic “lab-on-a-disc” platform. In: The 31st IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2018), 21-25 Jan, 2018, Belfast, Northern Ireland, UK,. ISBN ESSN: 2160-1968

This work presents a rotational-pulse actuated microfluidic cartridge enabling automated detection of plant pathogens on a compact device towards point-of-use monitoring of food safety. This highly integrated “Lab-on-a-Disc” (LoaD) system first runs the sample over a stationary phase of silica beads, followed by ethanol (EtOH) wash and final elution of DNA. The eluate is then homogenized using ‘shake mode’ agitation, accurately metered and then mixed with reagents for loop-mediated isothermal amplification (LAMP). We successfully purify plant DNA and demonstrate on-disc quantitative LAMP amplification.
Item Type:Conference or Workshop Item (Poster)
Event Type:Conference
Subjects:Biological Sciences > Microfluidics
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Physical Sciences
Publisher:IEEE Computer Society
Official URL:https://doi.org/10.1109/MEMSYS.2018.8346761
Copyright Information:©2018 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other users, including reprinting/ republishing this material for advertising or promotional purposes, creating new collective works for resale or redistribution to servers or lists, or reuse of any copyrighted components of this work in other works.
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
Funders:European Union (FP7-KBBE-2013-7-613908-DECATHLON, Science Foundation Ireland (SFI) and Fraunhofer Gesellschaft under its Strategic Partnership Programme Grant No.r 16/SPP/3321.
ID Code:22228
Deposited On:26 Jan 2018 14:41 by David Kinahan . Last Modified 30 Jul 2019 10:28

Full text available as:

[thumbnail of pg1134.pdf]
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader


Downloads per month over past year

Archive Staff Only: edit this record