Centrifugal blood sample preparation for metabolite derivatization and analysis by solid matrix laser desorption/ionization mass spectrometry (SMALDI-MS)
Hou, Yuting, Jing, Ji, Mishra, RohitORCID: 0000-0003-4279-4163, Ducrée, JensORCID: 0000-0002-0366-1897 and Harrison, D. Jed
(2018)
Centrifugal blood sample preparation for metabolite derivatization and analysis by solid matrix laser desorption/ionization mass spectrometry (SMALDI-MS).
In: Twenty Second International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2018), 11-15 Nov 2018., Kaohsiung, Taiwan.
A microfluidic approach is developed to automate and simplify multiplexed metabolite assays. Solid matrix assisted laser desorption ionization mass spectrometry (SMALDI-MS) on nanoporous thin films is suitable for analyzing small molecules, particularly metabolites, with a simple spot test. We present a system for blood sample preparation for metabolite analysis using centrifugal fluidics, coupled with derivatization and SMALDI-MS. This centrifugal microfluidic device enables semi-automated on-chip cleanup of blood samples, delivering a clean matrix for MS detection. A derivatization reagent is applied in our work to label the target molecules with fluorous affinity tags, so that higher S/N is observed by SMALDI-MS.
Metadata
Item Type:
Conference or Workshop Item (Poster)
Event Type:
Conference
Refereed:
Yes
Uncontrolled Keywords:
Centrifugal Microfluidics; Mass spectrometry; metabolite; nanoporous; whole blood