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Liposome immunoassay based on bio luminescent detection and its application to on-chip analysis

Nakatani, Yusuke, Maeki, Masatoshi orcid logoORCID: 0000-0001-7500-4231, Mishra, Rohit orcid logoORCID: 0000-0003-4279-4163, King, Damien, Ishida, Akihiko orcid logoORCID: 0000-0003-4100-9426, Ducrée, Jens orcid logoORCID: 0000-0002-0366-1897, Tani, Hirofumi orcid logoORCID: 0000-0002-5935-0756 and Tokeshi, Manabu orcid logoORCID: 0000-0002-4412-2144 (2015) Liposome immunoassay based on bio luminescent detection and its application to on-chip analysis. In: International Chemical Congress of Pacific Basin Societies (Pacifichem 2015, PAC CHEM2015), 19 Dec 2015, Honolulu, USA.

Luc-encapsulated liposomes were employed to develop on-chip immunoassays. Straight-channel chips were better than microbead for V-cup chips to immobilize several antibodies. Straight-channel chip that has PDMS flat substrate and wider channel (1000 μm) was optimum for this assay. To detect high BL intensity, fast flow rate (300 μl/min) was better than slower flow rate because channel was filled up with substrate quickly. However, substrate volume and lysis buffer didn't affect the detecttion high BL intensity. This implies that a sufficient amount of substrate was delivered to Luc in the liposomes. The LOD of CRP in this assay was 10 ng/mL.
Item Type:Conference or Workshop Item (Poster)
Event Type:Conference
Subjects:Biological Sciences > Biosensors
Biological Sciences > Microfluidics
Engineering > Biomedical engineering
Physical Sciences > Analytical chemistry
Physical Sciences > Nanotechnology
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Physical Sciences
Copyright Information:© 2015 the Authors
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
ID Code:24246
Deposited On:28 Feb 2020 10:11 by Rohit Mishra . Last Modified 28 Feb 2020 10:11

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