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Biosynthesis of gold nanoparticles by vascular cells in vitro

Kitching, Michael ORCID: 0000-0002-9884-0790, Inguva, Saikumar ORCID: 0000-0002-7648-3972, Ramani, Meghana ORCID: 0000-0001-7517-7618, Marsili, Enrico ORCID: 0000-0003-3150-1564 and Cahill, Paul A. ORCID: 0000-0002-5385-6502 (2022) Biosynthesis of gold nanoparticles by vascular cells in vitro. Frontiers in Microbiology . ISSN 1664-302X

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Abstract

Biosynthesis of gold nanoparticles (AuNPs) for antimicrobial and chemotherapeutic applications is a well-established process in microbial hosts such as bacterial, fungi, and plants. However, reports on AuNPs biosynthesis in mammalian cells are scarce. In this study, bovine aortic endothelial cells (BAECs) and bovine aortic smooth muscle cells (BASMCs) were examined for their ability to synthesize AuNPs in vitro. Cell culture conditions such as buffer selection, serum concentration, and HAuCl4 concentration were optimized before the biosynthesized AuNPs were characterized through visible spectrometry, transmission electron microscopy, X-ray diffraction, and Fourier transform infrared (FTIR) spectroscopy. BAECs and BASMC produced small, spherical AuNPs that are semi-crystalline with a similar diameter (23 ± 2 nm and 23 ± 4 nm). Hydrogen peroxide pretreatment increased AuNPs synthesis, suggesting that antioxidant enzymes may reduce Au3+ ions as seen in microbial cells. However, buthionine sulfoximine inhibition of glutathione synthesis, a key regulator of oxidative stress, failed to affect AuNPs generation. Taken together, these results show that under the right synthesis conditions, non-tumor cell lines can produce detectable concentrations of AuNPs in vitro.

Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:gold nanoparticles; biosynthesis; vascular cell; in vitro; biogenic nanoparticles
Subjects:Biological Sciences > Biochemistry
Biological Sciences > Cell biology
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology
DCU Faculties and Schools > Faculty of Science and Health > School of Physical Sciences
Publisher:Frontiers Media
Official URL:https://dx.doi.org/10.3389/fmicb.2022.81351
Copyright Information:© 2022 The Authors.
Funders:Irish Research Council Embark Initiative Ph.D. Scholarship, Collaborative Research Program 021220CRP0522, Nazarbayev University, Kazakhstan
ID Code:27798
Deposited On:27 Sep 2022 14:30 by Thomas Murtagh . Last Modified 23 Mar 2023 15:15

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