Cluster sizing of cancer cells by rail-based serial gap filtration in stopped-flow, continuous sedimentation mode
Glynn, MacdaraORCID: 0000-0002-6347-4348, Nwankire, Charles, Kinahan, David J.ORCID: 0000-0003-1968-2016 and Ducrée, JensORCID: 0000-0002-0366-1897
(2015)
Cluster sizing of cancer cells by rail-based serial gap filtration in stopped-flow, continuous sedimentation mode.
In: 28th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2015 ), 18-22 Jan 2015, Estoril, Portugal.
ISBN 978-1-4799-7955-4
In addition detecting circulating tumour cells (CTCs) in blood, the presence of multi-cellular clusters has recently been identified to carry further information pertaining to patient outcome. We present a label-free method of measuring the range and load of clusters in a blood sample using a size-exclusion rail operated by centrifugal microfluidics. A negative selection strategy first enriches clusters from a whole blood sample; these clusters are then processed along the rail where they resolve to a series of collection bins according to size. Analysis of the occupancy of these bins then provides metrics on the cancer-cell load carried in the blood.
Metadata
Item Type:
Conference or Workshop Item (Paper)
Event Type:
Conference
Refereed:
Yes
Uncontrolled Keywords:
Blood; Cells (biology); Rails; Microfluidics; Electron tubes; Cancer; Tumors;