Membranes in microfluidics have several uses, including separation/filtration such as particle separation, solute separation, and reverse osmosis. Membranes are increasingly being employed in biomedical applications such as blood partitioning and can be used to extract circulating tumor cells. In the current work, a femtosecond 400 fs laser was used to fabricate periodic structures on silicone membranes, including pCO2 and pO2. The goal is to generate periodic forms without cutting through the membranes’ thin coated surface. The maintenance of the hydrophobicity of the laser-fabricated samples is another area of interest in the study. To accomplish the study's goal, several input laser process parameters were varied. From the study, it was observed that the fabrication of different periodic structures on pCO2 and pO2 is feasible with the depth of penetration being controlled well via control of the scanning speed. This study provides a route for manufacturers to implement femtosecond laser surface profiling required for surface modification of medical and sensor membrane and products.