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Exploiting novel antibodies for the early detection of cardiac disease

Conroy, Paul John (2011) Exploiting novel antibodies for the early detection of cardiac disease. PhD thesis, Dublin City University.

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Abstract

Cardiovascular disease, the single greatest killer in the western world, is credited with causing 48% of all deaths worldwide and is of huge economic importance in healthcare provision. The cardiac troponins are definitive indicators of cardiovascular disease and elevated levels of cardiac troponin I (cTnI) are highly-specific for cardiovascular damage. Using a specific targeting strategy, a panel of novel antibodies were successfully generated against key epitopes in cTnI that are of paramount importance for specific cardiovascular disease detection. Panels of both monoclonal and recombinant antibodies were generated and fully characterised. This approach required a combination of high-throughput assay screening with „state-of-the-art‟ multiplexed surface-plasmon resonance-based instrumentation. The selected antibodies were demonstrated to outperform current commercially available reagents. In addition, inherent difficulties associated with the use and expression of cardiac troponin I, the key target analyte, were investigated in detail and alternative recombinant constructs developed and evaluated. The antibodies ultimately selected were chosen for their applicability for integration on to a point-of-care risk-chip for cardiovascular disease determination due to their specificity, high affinity and genetic tailoring for deployment on a microfluidic device. This stage involved close collaboration with clinical and industrial partners and the commercial feasibility and performance of the antibodies are now under evaluation.

Item Type:Thesis (PhD)
Date of Award:November 2011
Refereed:No
Supervisor(s):O'Kennedy, Richard
Uncontrolled Keywords:Cardiovascualr Disease; CVD; cardiac troponins
Subjects:Biological Sciences > Biotechnology
Biological Sciences > Molecular biology
Biological Sciences > Immunology
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology
Research Initiatives and Centres > Biomedical Diagnostics Institute (BDI)
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 License. View License
Funders:Irish Research Council for Science Engineering and Technology, Science Foundation Ireland
ID Code:16573
Deposited On:29 Nov 2011 16:00 by Richard O'Kennedy. Last Modified 29 Nov 2011 16:04

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