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Production of lectin-affinity matrices for process-scale glycoprotein purification

Thompson, Roisin and Keogh, Damien and O'Connell, Michael and Clarke, Paul A. and O'Connor, Brendan (2011) Production of lectin-affinity matrices for process-scale glycoprotein purification. In: Research Review Day 2011, 15 Jun 2011, DCU, Dublin, Ireland.

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Abstract

A selection of prokaryotic lectins with a variety of glycan specificities and affinities have been identified, cloned, expressed in Eschericia coli and characterised. The aims of this project are to: - express the lectins at 1L scale to produce sufficient quantities for immobilisation studies (~100 mg) - immobilisethelectinsonSepharose - evaluate lectin performance on column by monitoring their ability toreproducibly capture and elute glycoprotein glycoforms.

Item Type:Conference or Workshop Item (Poster)
Event Type:Conference
Refereed:Yes
Uncontrolled Keywords:lectins; lectin performance
Subjects:Biological Sciences > Biotechnology
Biological Sciences > Biochemistry
Biological Sciences > Molecular biology
DCU Faculties and Centres:Research Initiatives and Centres > Irish Separation Science Cluster (ISSC)
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
Funders:Science Foundation Ireland
ID Code:17768
Deposited On:08 Feb 2013 11:39 by Brendan O'Connor. Last Modified 15 Feb 2017 16:55

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