Production of lectin-affinity matrices for process-scale glycoprotein purification
Thompson, Roisin and Keogh, Damien and O'Connell, Michael and Clarke, Paul A. and O'Connor, Brendan (2011) Production of lectin-affinity matrices for process-scale glycoprotein purification. In: Research Review Day 2011, 15 Jun 2011, DCU, Dublin, Ireland.
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A selection of prokaryotic lectins with a variety of glycan specificities and affinities have been identified, cloned, expressed in Eschericia coli and characterised. The aims of this project are to:
- express the lectins at 1L scale to produce sufficient quantities for immobilisation studies (~100 mg)
- evaluate lectin performance on column by monitoring their ability toreproducibly capture and elute glycoprotein glycoforms.
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