The BETA-1, 3-Glucanase of Basidiomycete QM 806: Studies on it's production and application in yeast cell wall hydrolysis
Ryan, Eleanor M.
(1986)
The BETA-1, 3-Glucanase of Basidiomycete QM 806: Studies on it's production and application in yeast cell wall hydrolysis.
Master of Science thesis, Dublin City University.
In this project the growth and production of JB-1,3-glucanase by Basidiomycete sp QM 806 was investigated, with a view to studying its application in B-1,3-glucan degradation and in yeast extract production. The effect of various parameters on 3-1 »3-glucanase production was examined. The optimal conditions for enzyme production in submerged shake-flask culture were chosen.
Two B-1,3-glucans (laminarin and yeast cell walls) were
degraded using the B-1,3-glucanase produced. The
degradation products were identified using a high
performance liquid chromatographic method developed during
this project. The B-1,3-glucanase produced by Basidiomycete
sp QM 806 was compared with other commercially available
enzyme preparations, namely Kitalase and Novozym-234 which
were also used in B-1,3-glucan degradation experiments.
Yeast extract production experiments were conducted using
the Basidiomycete sp QM 806 culture supernatant, Kitalase
and Novozym-234. These experiments involved treating whole
baker's yeast cells (obtained commercially) with the various
enzyme preparations and calculating by dry weight the
percentage yeast extract produced. A comparison was drawn
between the enzyme preparations.