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Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Vaculovicova, Marketa and Akther, Mahbub and Maaskant, Pleun and Brabazon, Dermot (2015) Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems. Analytica Chimica Acta, 871 . pp. 85-92. ISSN 2584-7165

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Abstract

In this work, a new type of miniaturized fibre-coupled solid-state light source is demonstrated as an excitation source for fluorescence detection in capillary electrophoresis. It is based on a parabolically shaped micro- light emitting diode (µ-LED) array with a custom band-pass optical interference filter (IF) deposited at the back of the LED substrate. The GaN µ-LED array consisted of 270 individual µ-LED elements with peak emission at 470nm, each about 14µm in diameter and operated as a single unit. Light was extracted through the transparent substrate material, and coupled to an optical fibre (400µm in diameter, numerical aperture NA = 0.37), to form an integrated µ-LED-IF-OF light source component. This packaged µ-LED-IFOF light source emitted approximately 225µW of optical power at a bias current of 20mA. The bandpass IF filter was designed to reduce undesirable LED light emissions in the wavelength range above 490 nm . Devices with and without IF were compared in terms of optical power output, spectral characteristics as well as LOD values. While the IF consisted of only 7.5 pairs (15 layers) of SiO2/HfO2 layers it resulted in an improvement of the baseline noise as well as the detection limit measured using fluorescein as test analyte, both by approximately one order of magnitude, with a LOD of 1×10-8 mol/L obtained under optimised conditions. The µ-LED-IF-OF light source was then demonstrated for use in capillary electrophoresis with fluorimetric detection. Limits of detection obtained by this device were compared to those obtained with a commercial fibre coupled LED device.

Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:Micro-light emitting diode array; Light source; Optical fibre; Capillary electrophoresis; Fluorescence detection; Separations
Subjects:Engineering > Materials
Engineering > Mechanical engineering
DCU Faculties and Centres:Research Initiatives and Centres > Irish Separation Science Cluster (ISSC)
Research Initiatives and Centres > Advanced Processing Technology Research Centre (APTRC)
Publisher:Elsevier
Official URL:http://dx.doi.org/10.1016/j.aca.2015.02.044
Copyright Information:© 2015 Elsevier
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
ID Code:21075
Deposited On:01 Feb 2016 11:54 by Fran Callaghan. Last Modified 01 Apr 2016 01:02

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