Centrifugal automation of a highly customizable rapid-elisa test towards detection of cervical cancer in point-of-care settings
Mishra, RohitORCID: 0000-0003-4279-4163, Alam, Rizwan, McAuley, Darren, Bharaj, T, Kinahan, David J.ORCID: 0000-0003-1968-2016, Nwankire, Charles, Anderson, Karen and Ducrée, JensORCID: 0000-0002-0366-1897
(2016)
Centrifugal automation of a highly customizable rapid-elisa test towards detection of cervical cancer in point-of-care settings.
In: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences 9-13 October, 2016, Dublin, Ireland, 9-13 Oct 2016, Dublin, Ireland.
ISBN 978-0-9798064-9-0
Infections of high-risk strains of human papilloma virus (HPV16-E7) have been directly linked with cervical carcinogenesis [1]. Despite pervasive HPV testing and vaccinations, resource-poor regions continue to be affected. We present here a fully automated, bead-based Rapid Antigenic Protein In-situ Display (RAPID) Enzyme Linked Immunosorbent Assay (ELISA) for the detection of the onco-proteins of HPV16 E7 [1]. Advanced centrifugal flow control is implemented by a novel router based on lipophilic membranes in an event-trigger valving architecture [2]. By simply replacing the charge of RAPID-functionalized beads in the incubation chamber, the system may be customized for the detection of other biomarkers.