Centrifugal automation of a highly customizable rapid-elisa test towards detection of cervical cancer in point-of-care settings
Mishra, Rohit and Alam, Rizwan and McAuley, Darren and Bharaj, T and Kinahan, David J. and Nwankire, Charles and Anderson, Karen and Ducrée, Jens (2016) Centrifugal automation of a highly customizable rapid-elisa test towards detection of cervical cancer in point-of-care settings. In: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences 9-13 October, 2016, Dublin, Ireland, 9-13 Oct 2016, Dublin, Ireland. ISBN 978-0-9798064-9-0
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Infections of high-risk strains of human papilloma virus (HPV16-E7) have been directly linked with cervical carcinogenesis . Despite pervasive HPV testing and vaccinations, resource-poor regions continue to be affected. We present here a fully automated, bead-based Rapid Antigenic Protein In-situ Display (RAPID) Enzyme Linked Immunosorbent Assay (ELISA) for the detection of the onco-proteins of HPV16 E7 . Advanced centrifugal flow control is implemented by a novel router based on lipophilic membranes in an event-trigger valving architecture . By simply replacing the charge of RAPID-functionalized beads in the incubation chamber, the system may be customized for the detection of other biomarkers.
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