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Aldehyde dehydrogenase 1A1 and gelsolin identified as novel invasion-modulating factors in conditioned medium of pancreatic cancer cells

Walsh, Naomi and Dowling, Paul and O'Donovan, Norma and Henry, Michael and Meleady, Paula and Clynes, Martin (2008) Aldehyde dehydrogenase 1A1 and gelsolin identified as novel invasion-modulating factors in conditioned medium of pancreatic cancer cells. Journal of Proteomics, 71 (5). pp. 561-571. ISSN 1874-3919

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Abstract

Conditioned medium (CM) from clonal sub-populations of the pancreatic cancer cell line, MiaPaCa-2 with differing invasive abilities, were examined for their effect on in vitro invasion. Conditioned medium from Clone #3 (CM#3) strongly promoted invasion, while CM from Clone #8 (CM#8) inhibited invasion in vitro. 2D DIGE followed by MALDI-TOF MS analysis of CM#3 and CM#8 identified 41 proteins which were differentially regulated; 27 proteins were down-regulated and 14 proteins up-regulated in the invasion-promoting CM#3 when compared to CM#8. Western blotting analysis confirmed the down-regulated expression of gelsolin and the up-regulation of aldehyde dehydrogenase 1A1 in CM#3. Down-regulation of aldehyde dehydrogenase 1A1 in Clone #3 CM and gelsolin levels in Clone #8 CM by siRNA transfection revealed an important involvement of these proteins in promoting and inhibiting invasion in these pancreatic cancer cell lines.

Item Type:Article (Published)
Refereed:Yes
Subjects:Biological Sciences > Cell biology
Medical Sciences > Cancer
DCU Faculties and Centres:Research Initiatives and Centres > National Institute for Cellular Biotechnology (NICB)
Publisher:Elsevier
Official URL:http://dx.doi.org/10.1016/j.jprot.2008.09.002
Copyright Information:Copyright © 2008 Elsevier B.V.
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
Funders:This work was supported by the Irish Higher Education Authority's Programme for Research in Third-Level Institutions (PRTLI) cycle 3 and 4.
ID Code:2363
Deposited On:27 Jan 2009 17:07 by Naomi Walsh. Last Modified 27 Jan 2009 17:07

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