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Horseradish and soybean peroxidases: comparable tools for alternative niches?

Ryan, Barry J. and Carolan, Neil and Ó'Fágáin, Ciarán (2006) Horseradish and soybean peroxidases: comparable tools for alternative niches? Trends in Biotechnology, 24 (8). pp. 355-363. ISSN 0167-7799

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Abstract

Horseradish and soybean peroxidases (HRP and SBP, respectively) are useful biotechnological tools. HRP is often termed the classical plant heme peroxidase and although it has been studied for decades, our understanding has deepened since its cloning and subsequent expression, enabling numerous mutational and protein engineering studies. SBP, however, has been neglected until recently, despite offering a real alternative to HRP: SBP actually outperforms HRP in terms of stability and is now used in numerous biotechnological applications, including biosensors. Review of both is timely. This article summarizes and discusses the main insights into the structure and mechanism of HRP, with special emphasis on HRP mutagenesis, and outlines its use in a variety of applications. It also reviews the current knowledge and applications to date of SBP, particularly biosensors. The final paragraphs speculate on the future of plant heme-based peroxidases, with probable trends outlined and explored.

Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:horseradish peroxidase; soybean peroxidase review; biosensors; biocatalysis; hutagenesis
Subjects:Biological Sciences > Biotechnology
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology
Research Initiatives and Centres > National Centre for Sensor Research (NCSR)
Publisher:Elsevier
Official URL:http://dx.doi.org/10.1016/j.tibtech.2006.06.007
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
ID Code:79
Deposited On:26 Jun 2007 by DORAS Administrator. Last Modified 30 Jan 2009 14:10

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