A radio-over-fiber (RoF) distribution system incorporating both sub-carrier multiplexing and wavelength division multiplexing (WDM) technologies is presented. This signal is directly modulated onto three high-speed lasers. Bragg filters are employed at the receiver base station in order to both demultiplex the required optical channel, and ensure that the detected signal is single side band (in order to overcome dispersion limitations of the link). System spectral efficiency is optimised by wavelength interleaving. The channel spacing between the WDM channels is varied and the system performance for different values of channel spacing and spectral efficiencies is investigated. The results show that wavelength interleaving is a reliable technique that could be used to increase the spectral efficiency of RoF systems.