Genetically enhanced recombinant lectins for glyco-selective analysis and purification
Keogh, Damien, Larragy, Ruth, Thompson, Roisin, O'Connell, Michael, Clarke, Paul A. and O'Connor, BrendanORCID: 0000-0002-8999-5184
(2011)
Genetically enhanced recombinant lectins for glyco-selective analysis and purification.
In: Research Review Day 2011, 16 Jun 2011, DCU, Dublin, Ireland.
- Generation of a library of recombinant prokaryotic lectins (RPL’s) through random mutagenesis of the carbohydrate binding sites of bacterial lectins.
- Characterisation of mutant lectins with respect to structure and specificity
- Provision of mutant RPL’s with enhanced affinity and/or altered specificity, alongside wild-type RPL’s, for glycoprotein analysis and purification