Thompson, Roisin, Keogh, Damien, O'Connell, Michael, Clarke, Paul A. and O'Connor, Brendan 
ORCID: 0000-0002-8999-5184
(2011)
Production of lectin-affinity matrices for process-scale glycoprotein purification.
In: Research Review Day 2011, 15 Jun 2011, DCU, Dublin, Ireland.
Abstract
A selection of prokaryotic lectins with a variety of glycan specificities and affinities have been identified, cloned, expressed in Eschericia coli and characterised. The aims of this project are to:
- express the lectins at 1L scale to produce sufficient quantities for immobilisation studies (~100 mg)
- immobilisethelectinsonSepharose
- evaluate lectin performance on column by monitoring their ability toreproducibly capture and elute glycoprotein glycoforms.
Metadata
| Item Type: | Conference or Workshop Item (Poster) |
|---|---|
| Event Type: | Conference |
| Refereed: | Yes |
| Uncontrolled Keywords: | lectins; lectin performance |
| Subjects: | Biological Sciences > Biotechnology Biological Sciences > Biochemistry Biological Sciences > Molecular biology |
| DCU Faculties and Centres: | Research Institutes and Centres > Irish Separation Science Cluster (ISSC) |
| Use License: | This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License |
| Funders: | Science Foundation Ireland |
| ID Code: | 17768 |
| Deposited On: | 08 Feb 2013 11:39 by Brendan O'connor . Last Modified 03 Oct 2018 14:52 |
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