Probing the specific ion effects of biocompatible hydrated choline ionic liquids on lactate oxidase biofunctionality in sensor applications
Curto, Vincenzo F.ORCID: 0000-0002-0571-7903, Scheuermann, Stefan, Owens, RóisínORCID: 0000-0001-7856-2108, Ranganathan, Vijayaraghavan, MacFarlane, Douglas R., Benito-Lopez, FernandoORCID: 0000-0003-0699-5507 and Diamond, DermotORCID: 0000-0003-2944-4839
(2013)
Probing the specific ion effects of biocompatible hydrated choline ionic liquids on lactate oxidase biofunctionality in sensor applications.
Physical Chemistry Chemical, 2014
(16).
pp. 1841-1849.
ISSN 1463-9076
This paper presents an extended study on the ion effects of a series of biocompatible hydrated choline based ionic liquids (ILs) on lactate oxidase (LOx), an important enzyme in biosensing technology for the in vitro detection of lactic acid. Secondary structural analysis revealed changes in the protein conformation in hydrated ILs, while thermal unfolding/aggregation dynamics showed different profiles in the presence or absence of ILs. Moreover, LOx thermally denaturised at 90 1C showed residual activity in the presence of chloride and dihydrogen phosphate anions. Kinetic and lifetime studies were also performed, providing a better understanding of the ion effects of ILs on the biocatalytic activity of the enzyme.
This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
Funders:
VFC, SS, FBL, and DD acknowledge Science Foundation Ireland (grant 07/CE/I1147), SS is grateful to the Erasmus placement programme of the European Lifelong Learning Programme (LLP), RMO acknowledges funding from the European Research Council (ERC grant agreement no. 258966)., MASK, a Marie Curie International Research Staff Exchange Scheme
ID Code:
19709
Deposited On:
28 Jan 2014 14:26 by
Kevin Fraser
. Last Modified 05 May 2022 13:16