Centrifugally driven micro-fluidic discs (µ-CDs) have attracted significant interest within the analytical science community over the last decade, primarily being focused on the potential of such platforms for performing parallel and/or multiplex biological assays and further application in biomedical diagnostics. More recently, µ-CD based devices have also been applied to environmental analysis as platforms for multi-sample extraction and transportation, prior to off-disc analysis in the laboratory. Therefore, this review critically summarises recent developments with µ-CD platforms for sample extraction, preconcentration, fractionation and purification in bioanalytical and environmental applications. In addition to also summarising the 10 common methods employed in the fabrication of µCD platforms, as applications of µ-CDs in sample extraction are generally based on enclosed series of extraction phases/micro-columns, the preparation of these stationary phases in micro-fluidic channels embedded in µ-CDs is also discussed.