Given recent demands for more co-creational university technology commercialisation processes involving industry and end users, this paper adopts a micro level approach to explore the interaction between Quadruple Helix stakeholders within the university technology commercialisation process using a stakeholder lens. To explore this research question, a qualitative research methodology which relies upon comparative case analysis was adopted to explore the technology commercialisation process in two universities within a UK region. The findings revealed that university type impacts Quadruple Helix stakeholder salience and engagement and consequently university technology commercialisation activities and process. This is important as recent European regional policy fails to account for contextual influences when promoting Quadruple Helix stakeholder relationships in co-creational university technology commercialisation.