Kottelat, Jérémy ORCID: 0000-0002-9535-3612, Freeland, Brian ORCID: 0000-0003-3705-5745 and Dabros, Michal ORCID: 0000-0002-0176-0015 (2021) Novel strategy for the calorimetry-based control of fed-batch cultivations of saccharomyces cerevisiae. Processes, 9 (4). ISSN 2227-9717
Abstract
Typical controllers for fed-batch cultivations are based on the estimation and control of the specific growth rate in real time. Biocalorimetry allows one to measure a heat signal proportional to the substrate consumed by cells. The derivative of this heat signal is usually used to evaluate the specific growth rate, introducing noise to the resulting estimate. To avoid this, this study investigated a novel controller based directly on the heat signal. Time trajectories of the heat signal setpoint were modelled for different specific growth rates, and the controller was set to follow this dynamic setpoint. The developed controller successfully followed the setpoint during aerobic cultivations of Saccharomyces cerevisiae, preventing the Crabtree effect by maintaining low glucose concentrations. With this new method, fed-batch cultivations of S. cerevisiae could be reliably controlled at specific growth rates between 0.075 h−1 and 0.20 h−1, with average root mean square errors of 15 ± 3%.
Metadata
Item Type: | Article (Published) |
---|---|
Refereed: | Yes |
Additional Information: | Article number 723 |
Subjects: | Biological Sciences > Biotechnology Humanities > Biological Sciences > Biotechnology Biological Sciences > Food technology Humanities > Biological Sciences > Food technology Biological Sciences > Microbiology Humanities > Biological Sciences > Microbiology Engineering > Control theory |
DCU Faculties and Centres: | DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology |
Publisher: | MDPI |
Official URL: | https://doi.org/10.3390/pr9040723 |
Copyright Information: | © 2021 The Authors. |
ID Code: | 25788 |
Deposited On: | 21 Apr 2021 17:28 by Brian Freeland . Last Modified 21 Nov 2023 11:24 |
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