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Platelet-rich plasma releasate differently stimulates cellular commitment toward the chondrogenic lineage according to concentration

do Amaral, Ronaldo JFC orcid logoORCID: 0000-0002-2748-774X, Matsiko, Amos orcid logoORCID: 0000-0003-4740-5489, Tomazette, RP, Rocha, Wanessa KR, Cordeiro-Spinetti, Eric orcid logoORCID: 0000-0002-6840-2183, Levingstone, Tanya J. orcid logoORCID: 0000-0002-9751-2314, O'Brien, Fergal J. orcid logoORCID: 0000-0003-2030-8005, El-Cheikh, Marcia C. and Balduino, Alex orcid logoORCID: 0000-0002-6546-6974 (2015) Platelet-rich plasma releasate differently stimulates cellular commitment toward the chondrogenic lineage according to concentration. Journal of Tissue Engineering, 6 . ISSN 1552-4973

Platelet-rich plasma has been used to treat articular cartilage defects, with the expectations of anabolic and anti-inflammatory effects. However, its role on cellular chondrogenic or fibrogenic commitment is still a controversy. Herein, the role of platelet-rich plasma releasate, the product obtained following platelet-rich plasma activation, on cellular commitment toward the chondrogenic lineage was evaluated in vitro. Human nasoseptal chondrogenic cells and human bone marrow mesenchymal stromal cells were used as cell types already committed to the chondrogenic lineage and undifferentiated cells, respectively, as different concentrations of platelet-rich plasma releasate were tested in comparison to commonly used fetal bovine serum. Low concentration of platelet-rich plasma releasate (2.5%) presented similar effects on cellular growth compared to 10% fetal bovine serum, for both cell types. In a three-dimensional culture system, platelet-rich plasma releasate alone did not induce full nasoseptal chondrogenic cells cartilage-like pellet formation. Nonetheless, platelet-rich plasma releasate played a significant role on cell commitment as high-passage nasoseptal chondrogenic cells only originated cartilage-like pellets when expanded in the presence of platelet-rich plasma releasate rather than fetal bovine serum. Histological analyses and measurements of pellet area demonstrated that even low concentrations of platelet-rich plasma releasate were enough to prevent nasoseptal chondrogenic cells from losing their chondrogenic potential due to in vitro expansion thereby promoting their recommitment. Low concentration of platelet-rich plasma releasate supplemented in chondrogenic medium also increased the chondrogenic potential of mesenchymal stromal cells seeded on collagen-hyaluronic acid scaffolds, as observed by an increase in chondrogenic-related gene expression, sulfated glycosaminoglycan production, and compressive modulus following in vitro culture. On the contrary, higher concentration of platelet-rich plasma releasate (10%) hampered some of these features. In conclusion, platelet-rich plasma releasate was able to prevent cellular chondrogenic capacity loss, inducing regain of their phenotype, and modulate cell commitment. Our data support the hypothesis of platelet-rich plasma chondrogenic potential, allowing fetal bovine serum substitution for platelet-rich plasma releasate at specific concentrations in culture medium when chondrogenic commitment is desired on specific cell types and moments of culture.
Item Type:Article (Published)
Uncontrolled Keywords:Platelet-rich plasma; chondrogenesis; cartilage repair; mesenchymal stromal cells; nasoseptal chondrogenic cells
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Engineering and Computing > School of Mechanical and Manufacturing Engineering
Publisher:SAGE Publications
Official URL:https://dx.doi.org/10.1177/2041731415594127
Copyright Information:© 2015 The Authors.
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
Funders:Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq),, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Science Foundation Ireland, International Strategic Cooperation Award (12/ISCA/2494), and the Health Research Board of Ireland (HRA_POR/2011/27).
ID Code:27279
Deposited On:30 May 2022 14:08 by Thomas Murtagh . Last Modified 10 Jan 2023 15:05

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