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Siphon-controlled automation on a Lab-on-a-Disc using event-triggered dissolvable film valves

Henderson, Brian, Kinahan, David J. orcid logoORCID: 0000-0003-1968-2016, Rio, Jeanne, Mishra, Rohit orcid logoORCID: 0000-0003-4279-4163, King, Damien, Torres-Delgado, Sarai, Mager, Dario orcid logoORCID: 0000-0001-9487-3723, Korvink, Jan G. orcid logoORCID: 0000-0003-4354-7295 and Ducrée, Jens orcid logoORCID: 0000-0002-0366-1897 (2021) Siphon-controlled automation on a Lab-on-a-Disc using event-triggered dissolvable film valves. Biosensors, 11 (3). ISSN 2079-6374

Abstract
Within microfluidic technologies, the centrifugal microfluidic “Lab-on-a-Disc” (LoaD) platform offers great potential for use at the PoC and in low-resource settings due to its robustness and the ability to port and miniaturize ‘wet bench’ laboratory protocols. We present the combination of ‘event-triggered dissolvable film valves’ with a centrifugo-pneumatic siphon structure to enable control and timing, through changes in disc spin-speed, of the release and incubations of eight samples/reagents/wash buffers. Based on these microfluidic techniques, we integrated and automated a chemiluminescent immunoassay for detection of the CVD risk factor marker C-reactive protein displaying a limit of detection (LOD) of 44.87 ng mL−1 and limit of quantitation (LoQ) of 135.87 ng mL−1
Metadata
Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:lab-on-a-chip; centrifugal microfluidics; lab-on-a-disc; siphon valves; cardiovascular disease; chemiluminescence; immunoassa
Subjects:UNSPECIFIED
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Engineering and Computing > School of Mechanical and Manufacturing Engineering
DCU Faculties and Schools > Faculty of Science and Health > School of Physical Sciences
Research Institutes and Centres > National Centre for Sensor Research (NCSR)
Research Institutes and Centres > I-Form
Research Institutes and Centres > Water Institute
Publisher:MDPI
Official URL:https://dx.doi.org/10.3390/bios11030073
Copyright Information:© 2021 The Authors. Open Access (CC BY 4.0)
ID Code:27465
Deposited On:02 Aug 2022 13:01 by Thomas Murtagh . Last Modified 08 Aug 2022 17:18
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